European Commission logo
Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_FW377971
Database: nrnl1 Go to original record
Description: Transgenic plant event detection. Sequence 73 from Patent WO2008092866.
Taxon: unidentified
Original size: 97 bp
Patents: JP2010516270 (lens, espacenet) WO2008092866 (lens, espacenet)
Method: QL-ELE-00-020 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of cry1A(b) gene (Barbau-Piednoir et al., 2014).
Target:cryIAb

View other 267 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 13 85 - 73 bp
Amplicon: 73 bp 
1     accggttaca ctcccatcga catctccctc tccctcacgc agttcctgct
51    cagcgagttc gtgccaggtg ctg

View other 13 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 3.58e-09
primerB 100.0 19 bp 0 0 73 55 1.20e-08

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.