Match details
Record ID:
NRN_DJ058152
Database:
nrnl1
Go to original record
Description:
Corn Plant MON88017 and Compositions and Methods for Detection Thereof. Sequence 5 from patent US 8212113. JP 2012070734-A/5: Corn Plant MON88017 and Compositions and Methods for Detection Thereof.
Taxon:
unidentified
Original size:
7450 bp
Patents:
JP2007513641 (lens , espacenet )
US8212113 (lens , espacenet )
JP2012070734 (lens , espacenet )
View other 17 matches for this record
Method:
QL-CON-00-008
Go to GMOMETHODS record
View in JRC GMO-Matrix
Description: Qualitative PCR method for detection of the junction between the chloroplast transit peptide 2 and the CP4 epsps gene (Grohmann et al., 2009).
Target: CTP2-CP4 EPSPS
View other 257 matches for this method
Predicted amplification details:
Mismatches
Gaps
From
To
Strand
Amplicon size
0
0
1986
2073
+
88 bp
1 gggatgacgt taattggctc tgagcttcgt cctcttaagg tcatgtcttc
51 tgtttccacg gcgtgcatgc ttcacggtgc aagcagcc
View other 233 matches with an identical amplicon
Primers and probe alignment:
Primer/Probe
% Identity
Length
Mismatches
Gaps
From
To
Score
primerA
100.0
22 bp
0
0
1
22
3.82e-10
primerB
100.0
19 bp
0
0
88
70
1.47e-08
probe
100.0
27 bp
0
0
65
39
8.22e-13
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.