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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_EA059902
Database: nrnl1 Go to original record
Description: Sequence 6 from patent US 7166771. COORDINATED DECREASE AND INCREASE OF GENE EXPRESSION OF MORE THAN ONE GENE USING TRANSGENIC CONSTRUCTS. COORDINATED DECREASE AND INCREASE OF GENE EXPRESSION OF MORE THANONE GENE USING TRANSGENIC CONSTRUCTS. Sequence 6 from patent US 7795504.
Taxon: unidentified
Original size: 7713 bp
Patents: US7166771 (lens, espacenet) US2004126845 (lens, espacenet) JP2007506437 (lens, espacenet) US7795504 (lens, espacenet)

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Method: QL-ELE-00-001 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (BVL L 00.00-31, 1998).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
1 0 5957 6151 + 195 bp
Amplicon: 195 bp 
1     gcacctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat
51    gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
101   cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
151   gtgatatctc cactgacgta agggatgacg cacaatccca ctatc

View other 3571 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 94.7 19 bp 1 0 1 19 1.44e-06
primerB 100.0 20 bp 0 0 195 176 9.28e-09

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.