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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_CS195873
Database: nrnl1 Go to original record
Description: Sequence 1 from Patent WO2005103266. Sequence 1 from patent US 7179965. Sequence 1 from patent US 7883850. Sequence 1 from Patent EP2333082.
Taxon: unidentified
Original size: 15490 bp
Patents: WO2005103266 (lens, espacenet) US7179965 (lens, espacenet) US2005216969 (lens, espacenet) US7883850 (lens, espacenet) EP2333082 (lens, espacenet)

View other 5 matches for this record

Method: QT-EVE-GH-001a Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of cotton event 281-24-236 (Mazzara et al., 2006).
Target:DAS-24236-5

View other 11 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 12512 12622 + 111 bp
Amplicon: 111 bp 
1     ctcattgctg atccatgtag atttccctta cttgtctccc tctaatctga
51    ctttattaac ccaaagcaat tgcttatttg ttccccacgc ccacaaagcc
101   cagcattgtc c

View other 11 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 25 bp 0 0 1 25 1.24e-11
primerB 100.0 20 bp 0 0 111 92 5.57e-09
probe 100.0 33 bp 0 0 64 32 5.90e-16

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.