Match details

Record ID: NRN_CS158625
Database: nrnl1 Go to original record
Description: Sequence 13 from Patent WO2005080544. CELL/TISSUE CULTURING DEVICE, SYSTEM AND METHOD. PRODUCTION OF HIGH MANNOSE PROTEINS IN PLANT CULTURE. CELL/TISSUE CULTURING DEVICE, SYSTEM AND METHOD. PRODUCTION OF HIGH MANNOSE PROTEINS IN PLANT CULTURE. PRODUCTION OF HIGH MANNOSE PROTEINS IN PLANT CULTURE. Sequence 13 from patent US 7951557. Sequence 13 from Patent EP2220105. JP 2011184445-A/10: PRODUCTION OF HIGH MANNOSE PROTEINS IN PLANT CULTURE.
Taxon: unidentified
Original size: 2186 bp
Patents: WO2005080544 (lens, espacenet) JP2007522824 (lens, espacenet) JP2006524506 (lens, espacenet) JP2010525806 (lens, espacenet) US7951557 (lens, espacenet) EP2220105 (lens, espacenet) JP2011184445 (lens, espacenet)

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Method: QT-ELE-00-001 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (Feinberg et al., 2005).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 218 296 + 79 bp

Amplicon: 79 bp
1     cgtcttcaaa gcaagtggat tgatgtgata tctccactga cgtaagggat
51    gacgcacaat cccactatcc ttcgcaaga 

View other 6569 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 22 bp 0 0 1 22 3.41e-10
primerB 100.0 22 bp 0 0 79 58 3.41e-10
probe 100.0 26 bp 0 0 31 56 2.52e-12


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.