JRC GMO-Amplicons | EURL GMFF

Record ID:	NRN_CS457851
Database:	nrnl1	Go to original record
Description:	Sequence 23 from Patent WO2006122971. Sequence 23 from Patent WO2006122972. Sequence 23 from Patent WO2007134907. Sequence 23 from Patent WO2007134906. Treatment of Disease Using an Improved, Regulated Expression System.
Taxon:	unidentified
Original size:	6071 bp
Patents:	WO2006122971 (lens, espacenet) WO2006122972 (lens, espacenet) WO2007134907 (lens, espacenet) WO2007134907 (lens, espacenet) WO2007134906 (lens, espacenet) JP2008545639 (lens, espacenet)

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Method:	QL-ELE-00-003	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of Neomycin phosphotransferase II gene (BVL L 00.00-31, 1998)
Target:	nptII

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Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	4762	4934	+	173 bp

Amplicon:

173 bp

1     ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
51    ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
101   gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
151   cggtcttgtc gatcaggatg atc

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Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	16 bp	0	0	1	16	1.08e-06
primerB	100.0	16 bp	0	0	173	158	1.08e-06

Match details