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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_CQ924483
Database: nrnl1 Go to original record
Description: Sequence 10 from Patent EP1477557. Sequence 10 from patent US 6346655. Sequence 10 from patent US 6646184. Sequence 10 from patent US 7049421. Transgenic plant and methods. Transgenic plant resistant to mycotoxins and methods.
Taxon: unidentified
Original size: 13737 bp
Patents: EP1477557 (lens, espacenet) US6346655 (lens, espacenet) US6346655 (lens, espacenet) US6646184 (lens, espacenet) US2002162136 (lens, espacenet) JP2002540787 (lens, espacenet)

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Method: QL-CON-00-011 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of the junction between the Cauliflower Mosaic Virus 35S promoter and the pat gene
Target:CaMV P-35S-pat

View other 205 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 11340 11450 - 111 bp
Amplicon: 111 bp 
1     aagttcattt catttggaga ggacagggta cccggggatc ctctagagtc
51    gacatgtctc cggagaggag accagttgag attaggccag ctacagcagc
101   tgatatggcc g

View other 19 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 25 bp 0 0 1 25 1.24e-11
primerB 100.0 21 bp 0 0 111 91 1.65e-09
probe 100.0 28 bp 0 0 60 87 2.89e-13

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.