JRC GMO-Amplicons | EURL GMFF

Record ID:	NRN_CS566037
Database:	nrnl1	Go to original record
Description:	Sequence 24 from Patent WO2006039376. Sequence 24 from patent US 7323556. CORN EVENT DAS-59122-7 AND METHODS FOR DETECTION THEREOF. Sequence 24 from patent US 7695914. Sequence 24 from patent US 7696341. Sequence 24 from patent US 7875429. Sequence 24 from patent US 7875430. Sequence 24 from patent US 7888023. Sequence 24 from patent US 7888495. Sequence 24 from patent US 7897342. Sequence 24 from patent US 7932033. Sequence 24 from patent US 7956246. Sequence 1 from patent US 8273535. Sequence 1 from Patent WO2009100188.
Taxon:	unidentified
Original size:	7390 bp
Patents:	WO2006039376 (lens, espacenet) US7323556 (lens, espacenet) US2006070139 (lens, espacenet) JP2008514233 (lens, espacenet) US7695914 (lens, espacenet) US7696341 (lens, espacenet) US7875429 (lens, espacenet) US7875430 (lens, espacenet) US7888023 (lens, espacenet) US7888495 (lens, espacenet) US7897342 (lens, espacenet) US7932033 (lens, espacenet) US7956246 (lens, espacenet) US8273535 (lens, espacenet) WO2009100188 (lens, espacenet)

Method:	QL-CON-00-011	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of the junction between the Cauliflower Mosaic Virus 35S promoter and the pat gene
Target:	CaMV P-35S-pat

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	6317	6418	+	102 bp

Amplicon:

102 bp

1     aagttcattt catttggaga ggacagggta cccggggatc caccatgtct
51    ccggagagga gaccagttga gattaggcca gctacagcag ctgatatggc
101   cg

Primers and probe alignment:

Primer/Probe	% Identity	Length	From	To	Score
primerA	100.0	25 bp	1	25	1.13e-11
primerB	100.0	21 bp	102	82	1.51e-09
probe	100.0	28 bp	51	78	2.64e-13

Match details