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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_AX172477
Database: nrnl1 Go to original record
Description: Sequence 38 from Patent WO0141558. Sequence 38 from patent US 6506963. Sequence 38 from patent US 6563026. Sequence 38 from patent US 7659095. Sequence 38 from patent US 8026352. Sequence 38 from patent US 8309699.
Taxon: unidentified
Original size: 522 bp
Patents: WO0141558 (lens, espacenet) US6506963 (lens, espacenet) US6506963 (lens, espacenet) US6563026 (lens, espacenet) US2001029620 (lens, espacenet) US7659095 (lens, espacenet) US8026352 (lens, espacenet) US8309699 (lens, espacenet)
Method: QT-EVE-BN-002 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of oilseed rape event Ms8 (Mazzara et al., 2007).
Target:Ms8

View other 13 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 79 208 + 130 bp
Amplicon: 130 bp 
1     gttagaaaaa gtaaacaatt aatatagccg gctatttgtg taaaaatccc
51    taatataatc gacggatccc cgggaattcc gggggaagct tagatccatg
101   gatttgttat gataaccaaa aacaccctcc

View other 13 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 31 bp 0 0 1 31 8.34e-15
primerB 100.0 20 bp 0 0 130 111 6.57e-09
probe 100.0 28 bp 0 0 52 79 3.42e-13

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.