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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_AX342369
Database: nrnl1 Go to original record
Description: Sequence 8 from Patent EP1167531. Sequence 8 from Patent EP1582592. Sequence 8 from patent US 6825400. Sequence 8 from patent US 7193071. Sequence 8 from patent US 7582434. Sequence 8 from patent US 8273959. Sequence 8 from Patent EP2210951.
Taxon: unidentified
Original size: 1183 bp
Patents: EP1167531 (lens, espacenet) EP1582592 (lens, espacenet) US6825400 (lens, espacenet) US2002013960 (lens, espacenet) US7193071 (lens, espacenet) US2004139493 (lens, espacenet) US7582434 (lens, espacenet) US2007056056 (lens, espacenet) US8273959 (lens, espacenet) EP2210951 (lens, espacenet)

View other 1 match for this record

Method: QL-ELE-00-018 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of nopaline synthase terminator (Barbau-Piednoir et al., 2014).
Target:T-nos

View other 15568 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
1 0 28 96 + 69 bp
Amplicon: 69 bp 
1     gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat
51    agcgcgcaaa ctaggataa

View other 15381 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 28 bp 0 0 1 28 1.84e-13
primerB 96.0 25 bp 1 0 69 45 9.65e-11

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.