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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_EA135634
Database: nrnl1 Go to original record
Description: Sequence 12 from patent US 7223907.
Taxon: unidentified
Original size: 1360 bp
Patents: US7223907 (lens, espacenet) US2004250317 (lens, espacenet)

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Method: QT-EVE-GH-005 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of cotton event MON15985 (Savini et al., 2008).
Target:MON-15985-7

View other 2 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 299 380 + 82 bp
Amplicon: 82 bp 
1     gttactagat cggggatatc cccggggcgg ccgctctaga actagtggat
51    ctgcactgaa atcccatcca tttagcaacc tt

View other 2 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 1.20e-09
primerB 100.0 21 bp 0 0 82 62 1.20e-09
probe 100.0 30 bp 0 0 31 60 1.86e-14

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.