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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_AX172469
Database: nrnl1 Go to original record
Description: Sequence 30 from Patent WO0141558. Sequence 30 from patent US 6506963. Sequence 30 from patent US 6563026. Sequence 30 from patent US 7659095. Sequence 30 from patent US 8026352. Sequence 30 from patent US 8309699.
Taxon: unidentified
Original size: 1501 bp
Patents: WO0141558 (lens, espacenet) US6506963 (lens, espacenet) US6506963 (lens, espacenet) US6563026 (lens, espacenet) US2001029620 (lens, espacenet) US7659095 (lens, espacenet) US8026352 (lens, espacenet) US8309699 (lens, espacenet)
Method: QT-EVE-BN-003 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of oilseed rape event Rf3 (Savini et al., 2007).
Target:Rf3

View other 6 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 86 224 + 139 bp
Amplicon: 139 bp 
1     cataaaggaa gatggagact tgagaagttt tttttggact ttgtttagct
51    ttgttgggcg tttttttttt ttgatcaata actttgttgg gcttatggtc
101   gataagcgtg cgcatgtctg atggtacatg ctaaatgct

View other 6 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 24 bp 0 0 1 24 5.02e-11
primerB 100.0 25 bp 0 0 139 115 1.47e-11
probe 100.0 25 bp 0 0 112 88 1.47e-11

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.