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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_AR110602
Database: nrnl1 Go to original record
Description: Sequence 9 from patent US 6114608. Sequence 9 from patent US 6229075. Sequence 9 from patent US 6232533. Sequence 9 from patent US 6232534. Sequence 9 from patent US 6342660. Sequence 9 from patent US 6399860. Sequence 9 from patent US 6573438.
Taxon: unidentified
Original size: 7378 bp
Patents: US6114608 (lens, espacenet) US6229075 (lens, espacenet) US6229075 (lens, espacenet) US6232533 (lens, espacenet) US6232533 (lens, espacenet) US6232534 (lens, espacenet) US6232534 (lens, espacenet) US6342660 (lens, espacenet) US6342660 (lens, espacenet) US6399860 (lens, espacenet) US6399860 (lens, espacenet) US6573438 (lens, espacenet) US6573438 (lens, espacenet)

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Method: QL-ELE-00-009 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of nopaline synthase terminator (Lipp et al.,2001).
Target:T-nos

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 2996 3113 + 118 bp
Amplicon: 118 bp 
1     gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
51    tacatttaat acgcgataga aaacaaaata tagcgcgcaa cctaggataa
101   attatcgcgc gcggtgtc

View other 60 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 24 bp 0 0 1 24 4.22e-11
primerB 100.0 24 bp 0 0 118 95 4.22e-11

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.