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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_AX195447
Database: nrnl1 Go to original record
Description: Sequence 10 from Patent WO0151654. Sequence 10 from patent US 6395485. Sequence 10 from patent US 6951929. Methods and kits for identifying elite event GAT-ZM1 in biological samples.
Taxon: unidentified
Original size: 484 bp
Patents: WO0151654 (lens, espacenet) US6395485 (lens, espacenet) US6395485 (lens, espacenet) US6951929 (lens, espacenet) US2001029014 (lens, espacenet) JP2003524416 (lens, espacenet)

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Method: QT-EVE-ZM-011 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of maize event T25 (Mazzara et al., 2013).
Target:T25

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 286 387 + 102 bp
Amplicon: 102 bp 
1     acaagcgtgt cgtgctccac catgttgacg aagattttct tcttgtcatt
51    gagtcgttcc gccattgtcg ctgtcgcacg gcggtggaag gagtatcatg
101   tc

View other 4 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 5.10e-09
primerB 100.0 21 bp 0 0 102 82 1.51e-09
probe 100.0 25 bp 0 0 46 70 1.13e-11

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.