Match details

Record ID: NRN_BD014475
Database: nrnl1 Go to original record
Description: Transgenic corn and method of detecting recombinant gene from foods containing the same.
Taxon: unidentified
Original size: 886 bp
Patents: JP2001136983 (lens, espacenet)

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Method: QT-ELE-00-003 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of synthetic cry1A(b) gene (ISO/FDIS 21570:2005).
Target:cryIAb

View other 199 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 651 779 + 129 bp

Amplicon: 129 bp
1     cccatcgaca tcagcctgag cctgacccag ttcctgctga gcgagttcgt
51    gcccggcgcc ggcttcgtgc tgggcctggt ggacatcatc tggggcatct
101   tcggccccag ccagtgggac gccttcctg 

View other 183 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 1.93e-09
primerB 100.0 20 bp 0 0 129 110 6.52e-09
probe 100.0 21 bp 0 0 86 66 1.93e-09


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.