Match details
Record ID: |
NRN_BD014475 |
Database: |
nrnl1 |
Go to original record |
Description: |
Transgenic corn and method of detecting recombinant gene from foods containing the same. |
Taxon: |
unidentified |
Original size: |
886 bp |
Patents: |
JP2001136983 (lens, espacenet)
|
View other 1 match for this record
Method: |
QL-CON-00-004 |
Go to GMOMETHODS record |
|
View in JRC GMO-Matrix
|
Description: | Qualitative PCR method for detection of the junction between the CDPK promoter from maize and the synthetic cry1A(b) gene (ISO/FDIS 21569:2005). |
Target: | P-CDPK-cryIAb |
View other 1 match for this method
Predicted amplification details: |
Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
0 |
0 |
466 |
677 |
+ |
212 bp |
|
1 ctctcgccgt tcatgtccgt ggctggctgc cctccgtggg agcaggcggc
51 cgcactcgtt ccccgccgca gccggatcca acaatggaca acaaccccaa
101 catcaacgag tgcatcccct acaactgcct gagcaacccc gaggtggagg
151 tgctgggcgg cgagcgcatc gagaccggct acacccccat cgacatcagc
201 ctgagcctga cc
View other 1 match with an identical amplicon
Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
primerA |
100.0 |
20 bp |
0 |
0 |
1 |
20 |
1.01e-08 |
primerB |
100.0 |
20 bp |
0 |
0 |
212 |
193 |
1.01e-08 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.