Match details

Record ID: NRN_BD014475
Database: nrnl1 Go to original record
Description: Transgenic corn and method of detecting recombinant gene from foods containing the same.
Taxon: unidentified
Original size: 886 bp
Patents: JP2001136983 (lens, espacenet)

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Method: QL-CON-00-004 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of the junction between the CDPK promoter from maize and the synthetic cry1A(b) gene (ISO/FDIS 21569:2005).
Target:P-CDPK-cryIAb

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 466 677 + 212 bp

Amplicon: 212 bp
1     ctctcgccgt tcatgtccgt ggctggctgc cctccgtggg agcaggcggc
51    cgcactcgtt ccccgccgca gccggatcca acaatggaca acaaccccaa
101   catcaacgag tgcatcccct acaactgcct gagcaacccc gaggtggagg
151   tgctgggcgg cgagcgcatc gagaccggct acacccccat cgacatcagc
201   ctgagcctga cc 

View other 1 match with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 1.01e-08
primerB 100.0 20 bp 0 0 212 193 1.01e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.