Match details

Record ID: NRN_GM037889
Database: nrnl1 Go to original record
Description: Sequence 5 from Patent WO2008054747.
Taxon: unidentified
Original size: 39499 bp
Patents: WO2008054747 (lens, espacenet)

Method: QT-EVE-GM-008 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of soybean event 305423 (Mazzara et al., 2009).
Target:DP-305423-1

View other 5 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 31555 31647 + 93 bp

Amplicon: 93 bp
1     cgtgttctct ttttggctag ctagtgtttt tttctcgact tttgtatgaa
51    aatcatttgt gtcaatagtt tgtgttatgt attcattggt cac 

View other 5 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 1.37e-09
primerB 100.0 28 bp 0 0 93 66 2.53e-13
probe 100.0 32 bp 0 0 64 33 1.69e-15


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.