Match details
Record ID:
NRN_AX033493
Database:
nrnl1
Go to original record
Description:
Sequence 1 from Patent DE19906169. Test kit and method for quantitatively detecting genetically modi-fied DNA in foodstuff by means of fluorescence-coupled PCR.
Taxon:
unidentified
Original size:
240 bp
Patents:
DE19906169 (lens , espacenet )
JP2002536024 (lens , espacenet )
View other 3 matches for this record
Method:
QL-CON-00-006
Go to GMOMETHODS record
View in JRC GMO-Matrix
Description: Qualitative PCR method for detection of the junction between the CaMV35S promoter and the chloroplast transit peptide sequence (EU-Project SMT4-CT96-2072:1998).
Target: CaMV P-35S-CTP
View other 9 matches for this method
Predicted amplification details:
Mismatches
Gaps
From
To
Strand
Amplicon size
0
0
20
190
+
171 bp
1 tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
51 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga
101 cacgctgaca agctgactct agcagatctt tcaagaatgg cacaaattaa
151 caacatggct caagggatac a
View other 5 matches with an identical amplicon
Primer/Probe
% Identity
Length
Mismatches
Gaps
From
To
Score
primerA
100.0
22 bp
0
0
1
22
7.16e-10
primerB
100.0
22 bp
0
0
171
150
7.16e-10
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.