Match details

Record ID: NRN_FB706319
Database: nrnl1 Go to original record
Description: Sequence 26 from Patent WO2007127381. MUTANT DELTA-8 DESATURASE GENES ENGINEERED BY TARGETED MUTAGENSIS AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS. Sequence 68 from Patent WO2008063340. Sequence 185 from Patent WO2008073271. Sequence 185 from Patent WO2008073275. Sequence 185 from patent US 7709239. Sequence 68 from patent US 7863502. Sequence 26 from patent US 7943823. Sequence 185 from patent US 8017838. Sequence 185 from patent US 8026089. Sequence 26 from patent US 8058517. Sequence 26 from Patent EP2405012.
Taxon: unidentified
Original size: 7887 bp
Patents: WO2007127381 (lens, espacenet) WO2007127381 (lens, espacenet) JP2010512146 (lens, espacenet) WO2008063340 (lens, espacenet) WO2008073271 (lens, espacenet) WO2008073275 (lens, espacenet) US7709239 (lens, espacenet) US7863502 (lens, espacenet) US7943823 (lens, espacenet) US8017838 (lens, espacenet) US8026089 (lens, espacenet) US8058517 (lens, espacenet) EP2405012 (lens, espacenet)

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Method: QL-ELE-00-012 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative duplex PCR method for detection of Cauliflower Mosaic Virus 35S promoter and nopaline synthase terminator (partim CaMV P-35S) (Waiblinger et al., 2008).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 3105 3186 + 82 bp

Amplicon: 82 bp
1     gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
51    cgtggaaaaa gaagacgttc caaccacgtc tt 

View other 26489 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 18 bp 0 0 1 18 4.54e-08
primerB 100.0 22 bp 0 0 82 61 3.55e-10
probe 100.0 22 bp 0 0 21 42 3.55e-10


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.