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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: KT184681.1
Database: emblreltgn Go to original record
Description: Zea mays transgenic strain MON863e promoter region.
Taxon: Zea mays
Original size: 937 bp

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Method: QT-EVE-ZM-009 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of maize event MON863 (Mazzara et al., 2005).
Target:MON 863

View other 3 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 692 775 + 84 bp
Amplicon: 84 bp 
1     tgttacggcc taaatgctga actattgacc ctacttgttc ggatgggtgt
51    tcaccccaaa gtgtaccaag ctttccgatc ctac

View other 3 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 23 bp 0 0 1 23 1.07e-10
primerB 100.0 22 bp 0 0 84 63 3.64e-10
probe 100.0 28 bp 0 0 53 26 2.28e-13

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.