Match details
Record ID:
EU880444.1
Database:
emblreltgn
Go to original record
Description:
Oryza sativa Indica Group transgenic insect-resistant line TT51-1 integrated transgenes and flanking genomic sequence.
Taxon:
Oryza sativa Indica Group
Original size:
10536 bp
View other 39 matches for this record
Method:
QL-CON-00-009
Go to GMOMETHODS record
View in JRC GMO-Matrix
Description: Qualitative PCR method for detection of the junction between cry1A(b)/cry1A(c) and DNA spacer sequences in the context of Commission Decision 2008/289/EC)
Target: cry1Ab-T-NOS
View other 3 matches for this method
Predicted amplification details:
Mismatches
Gaps
From
To
Strand
Amplicon size
0
0
7167
7249
+
83 bp
1 gactgctgga gtgattatcg acagattcga gttcattcca gttactgcaa
51 cactcgaggc tgaataagtc gaggtaccga gct
View other 7 matches with an identical amplicon
Primers and probe alignment:
Primer/Probe
% Identity
Length
Mismatches
Gaps
From
To
Score
primerA
100.0
25 bp
0
0
1
25
9.09e-12
primerB
100.0
24 bp
0
0
83
60
3.11e-11
probe
100.0
32 bp
0
0
27
58
1.58e-15
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.