Match details
| Record ID: |
EU880444.1 |
| Database: |
emblreltgn |
Go to original record |
| Description: |
Oryza sativa Indica Group transgenic insect-resistant line TT51-1 integrated transgenes and flanking genomic sequence. |
| Taxon: |
Oryza sativa Indica Group |
| Original size: |
10536 bp |
View other 39 matches for this record
| Method: |
QL-CON-00-007 |
Go to GMOMETHODS record |
|
View in JRC GMO-Matrix
|
|
| Description: | Qualitative PCR method for detection of the junction between a cry1A(b)/cry1A(c) fusion gene and DNA spacer sequences (Grohmann and Maede, 2009). |
| Target: | cry1Ab-T-NOS |
View other 3 matches for this method
| Predicted amplification details: |
| Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
| 0 |
0 |
2825 |
2907 |
+ |
83 bp |
|
1 gactgctgga gtgattatcg acagattcga gttcattcca gttactgcaa
51 cactcgaggc tgaataagtc gaggtaccga gct
View other 7 matches with an identical amplicon
| Primers and probe alignment: |
| Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
| primerA |
100.0 |
25 bp |
0 |
0 |
1 |
25 |
9.09e-12 |
| primerB |
100.0 |
24 bp |
0 |
0 |
83 |
60 |
3.11e-11 |
| probe |
100.0 |
32 bp |
0 |
0 |
27 |
58 |
1.58e-15 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.
