Match details
| Record ID: |
EU124676.1 |
| Database: |
emblreltgn |
Go to original record |
| Description: |
Brassica napus transgenic cultivar Topas 19/2 3'-terminal transgene junction sequence. |
| Taxon: |
Brassica napus |
| Original size: |
823 bp |
View other 5 matches for this record
| Method: |
QT-EVE-BN-008 |
Go to GMOMETHODS record |
|
View in JRC GMO-Matrix
|
|
| Description: | Quantitative PCR method for detection of oilseed rape event Topas 19/2 (verified by the EU-RL GMFF in the context of Commission Decision 2007/307/EC) |
| Target: | Topas 19/2 |
View other 1 match for this method
| Predicted amplification details: |
| Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
| 0 |
0 |
478 |
572 |
+ |
95 bp |
|
1 gttgcggttc tgtcagttcc aaacgtaaaa cggcttgtcc cgcgtcatcg
51 gcgggggtcg taacgtgact cccggtcata tatcagcgcc ggtcg
View other 1 match with an identical amplicon
| Primers and probe alignment: |
| Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
| primerA |
100.0 |
20 bp |
0 |
0 |
1 |
20 |
4.73e-09 |
| primerB |
100.0 |
20 bp |
0 |
0 |
95 |
76 |
4.73e-09 |
| probe |
100.0 |
17 bp |
0 |
0 |
38 |
54 |
1.76e-07 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.
