Match details

Record ID: GV611446.1
Database: emblrelpat Go to original record
Description: Sequence 43 from patent US 7635799.
Taxon: unidentified
Original size: 3304 bp
Patents: US7635799 (lens, espacenet)

Method: QT-EVE-ZM-019 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of maize event 3272 (Charles Delobel et al., 2008).
Target:SYN-E3272-5

View other 7 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 218 312 + 95 bp

Amplicon: 95 bp
1     tcatcagacc agattctctt ttatggccgg ccggccggcc ctgctgactg
51    ctgacgcggc caaacactga tagtttaaac tgaaggcggg aaacg 

View other 7 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 26 bp 0 0 1 26 3.06e-12
primerB 100.0 20 bp 0 0 95 76 4.73e-09
probe 100.0 23 bp 0 0 47 69 1.22e-10


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.