Match details

Record ID: GP755645.1
Database: emblrelpat Go to original record
Description: Sequence 42 from patent US 7619146.
Taxon: unidentified
Original size: 3328 bp
Patents: US7619146 (lens, espacenet)

Method: QL-ELE-00-024 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of tE9 terminator (Debode et al., 2016).
Target:tE9

View other 1054 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 3173 3259 - 87 bp

Amplicon: 87 bp
1     tttttattcg gttttcgcta tcgaactgtg aaatggaaat ggatggagaa
51    gagttaatga atgatatggt ccttttgttc attctca 

View other 1026 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 23 bp 0 0 1 23 1.11e-10
primerB 100.0 26 bp 0 0 87 62 2.79e-12
probe 100.0 36 bp 0 0 60 25 1.09e-17


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.