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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: AX127757.1
Database: emblrelpat Go to original record
Description: Sequence 10 from Patent WO0131042.
Taxon: synthetic construct
Original size: 416 bp
Patents: WO0131042 (lens, espacenet)
Method: QT-EVE-BN-011 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of oilseed rape event MS11 (Jacchia et al., 2019).
Target:BCS-BN012-7

View other 13 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 141 263 + 123 bp
Amplicon: 123 bp 
1     caagatggga attaacatct acaaattgcc ttttcttatc gaccatgtac
51    atcctaccat aattataatt ataattatat aatactgaaa ccatggtgcc
101   ccctgctgct ttacatggat ttc

View other 6 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 28 bp 0 0 1 28 3.22e-13
primerB 100.0 23 bp 0 0 123 101 1.60e-10
probe 100.0 20 bp 0 0 40 59 6.21e-09

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.