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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: AX093003.1
Database: emblrelpat Go to original record
Description: Sequence 8 from Patent WO0118192.
Taxon: synthetic construct
Original size: 6767 bp
Patents: WO0118192 (lens, espacenet)

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Method: QL-CON-00-014 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of the junction between the nos promoter and the neomycin phosphotransferase II gene
Target:P-nos-nptII

View other 1572 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 5424 5587 - 164 bp
Amplicon: 164 bp 
1     ttcccctcgg tatccaatta gagtctcata tttactctca atccaaataa
51    tctgcatccg gatctggatc gtttcgcatg attgaacaag atggattgca
101   cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
151   cacaacagac aatc

View other 35 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 23 bp 0 0 1 23 2.02e-10
primerB 100.0 22 bp 0 0 164 143 6.85e-10
probe 100.0 25 bp 0 0 141 117 1.75e-11

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.