European Commission logo
Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: AX092999.1
Database: emblrelpat Go to original record
Description: Sequence 4 from Patent WO0118192.
Taxon: synthetic construct
Original size: 6016 bp
Patents: WO0118192 (lens, espacenet)

View other 9 matches for this record

Method: QL-CON-00-014 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of the junction between the nos promoter and the neomycin phosphotransferase II gene
Target:P-nos-nptII

View other 1572 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 5424 5586 - 163 bp
Amplicon: 163 bp 
1     ttcccctcgg tatccaatta gagtctcata tttactctca atccaaataa
51    tctgcaccgg atctggatcg tttcgcatga ttgaacaaga tggattgcac
101   gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
151   acaacagaca atc

View other 11 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 23 bp 0 0 1 23 2.01e-10
primerB 100.0 22 bp 0 0 163 142 6.81e-10
probe 100.0 25 bp 0 0 140 116 1.74e-11

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.