Match details

Record ID: AX033493.1
Database: emblrelpat Go to original record
Description: Sequence 1 from Patent DE19906169.
Taxon: synthetic construct
Original size: 240 bp
Patents: DE19906169 (lens, espacenet)

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Method: QL-CON-00-001 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of the junction between the CaMV35S promoter and the chloroplast transit peptide sequence (ISO/FDIS 21569:2005).
Target:CaMV P-35S-CTP

View other 9 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 20 190 + 171 bp

Amplicon: 171 bp
1     tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
51    ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga
101   cacgctgaca agctgactct agcagatctt tcaagaatgg cacaaattaa
151   caacatggct caagggatac a 

View other 5 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 22 bp 0 0 1 22 7.16e-10
primerB 100.0 22 bp 0 0 171 150 7.16e-10


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.