Match details

Record ID: DI119995.1
Database: emblrelpat Go to original record
Description: ISOFORMS OF EIF-5A: SENESCENCE-INDUCED EIF5A;WOUNDING-INDUCED EIF-5A; GROWTH EIF-5A; AND DHS.
Taxon: unidentified
Original size: 662 bp

Method: QL-ELE-00-024 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of tE9 terminator (Debode et al., 2016).
Target:tE9

View other 1054 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 170 257 + 88 bp

Amplicon: 88 bp
1     tttttattcg gttttcgcta tcgaactgtg caaatggaaa tggatggaga
51    agagttaatg aatgatatgg tccttttgtt cattctca 

View other 10 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 23 bp 0 0 1 23 1.13e-10
primerB 100.0 26 bp 0 0 88 63 2.82e-12
probe 97.3 37 bp 0 1 61 25 5.12e-16


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.