Match details

Record ID:	A76915.1
Database:	emblrelpat	Go to original record
Description:	Sequence 1 from Patent EP0757102.
Taxon:	Transformation vector pTHW107
Original size:	4946 bp
Patents:	EP0757102 (lens, espacenet)

View other 8 matches for this record

Method:	QL-ELE-00-026	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative duplex PCR method for detection of pat gene; bar gene (partim bar) (Debode et al., 2016).
Target:	bar

View other 2622 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	733	792	-	60 bp

Amplicon:

60 bp

1     acaagcacgg tcaacttccg taccgagccg caggaaccgc aggagtggac
51    ggacgacctc

View other 5085 matches with an identical amplicon

Primers and probe alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	19 bp	0	0	1	19	1.06e-08
primerB	100.0	18 bp	0	0	60	43	3.56e-08
probe	100.0	18 bp	0	0	21	38	3.56e-08

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.