Match details

Record ID: BD105559.1
Database: emblrelpat Go to original record
Description: Method of constructing transgenic plant accompanied by biosynthesis of modified 5-aminolevulinic acid and method of identifying 5-aminolevulinic acid synthesis effector.
Taxon: unidentified
Original size: 4186 bp
Patents: JP2001526041 (lens, espacenet)

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Method: QL-CON-00-005 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of the junction between the pat gene and the CaMV35S terminator (ISO/FDIS 21569:2005).
Target:pat-CaMV T-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 345 535 - 191 bp

Amplicon: 191 bp
1     atggtggatg gcatgatgtt ggtttttggc aaagggattt tgagttgcca
51    gctcctccaa ggccagttag gccagttacc cagatctgac gctgaaatca
101   ccagtctctc tctacaaatc tatctctctc tataataatg tgtgagtagt
151   tcccagataa gggaattagg gttcttatag ggtttcgctc a 

View other 3 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 2.71e-09
primerB 100.0 23 bp 0 0 191 169 2.37e-10


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.