Match details

Record ID: AR606321.1
Database: emblrelpat Go to original record
Description: Sequence 1 from patent US 6818807.
Taxon: unidentified
Original size: 850 bp
Patents: US6818807 (lens, espacenet)

Method: QT-EVE-GH-002 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of cotton event LLCotton25 (Mazzara et al., 2007).
Target:LLCotton25

View other 6 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 653 730 + 78 bp

Amplicon: 78 bp
1     caaggaacta ttcaactgag cttaacagta ctcggccgtc gaccgcggta
51    cccggaattc caatcccaca aaaatctg 

View other 4 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 3.84e-09
primerB 100.0 24 bp 0 0 78 55 2.91e-11
probe 100.0 26 bp 0 0 21 46 2.48e-12


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.