Match details

Record ID:	AR491631.1
Database:	emblrelpat	Go to original record
Description:	Sequence 5 from patent US 6716474.
Taxon:	unidentified
Original size:	10846 bp
Patents:	US6716474 (lens, espacenet)

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Method:	QL-ELE-00-024	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of tE9 terminator (Debode et al., 2016).
Target:	tE9

View other 1054 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1862	1948	+	87 bp

Amplicon:

87 bp

1     tttttattcg gttttcgcta tcgaactgtg aaatggaaat ggatggagaa
51    gagttaatga atgatatggt ccttttgttc attctca

View other 1026 matches with an identical amplicon

Primers and probe alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	23 bp	0	0	1	23	1.11e-10
primerB	100.0	26 bp	0	0	87	62	2.79e-12
probe	100.0	36 bp	0	0	60	25	1.09e-17

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.