Match details

Record ID:	AR490568.1
Database:	emblrelpat	Go to original record
Description:	Sequence 3 from patent US 6713259.
Taxon:	unidentified
Original size:	566 bp
Patents:	US6713259 (lens, espacenet)

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Method:	QT-EVE-ZM-020	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Quantitative PCR method for detection of maize event MON810.
Target:	MON 810

View other 6 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	208	299	+	92 bp

Amplicon:

92 bp

1     tcgaaggacg aaggactcta acgtttaaca tcctttgcca ttgcccagct
51    atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gc

View other 13 matches with an identical amplicon

Primers and probe alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	24 bp	0	0	1	24	3.46e-11
primerB	100.0	24 bp	0	0	92	69	3.46e-11
probe	100.0	23 bp	0	0	27	49	1.18e-10

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.