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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: AR225313.1
Database: emblrelpat Go to original record
Description: Sequence 5 from patent US 6441277.
Taxon: unidentified
Original size: 10846 bp
Patents: US6441277 (lens, espacenet)

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Method: QL-CON-00-008 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of the junction between the chloroplast transit peptide 2 and the CP4 epsps gene (Grohmann et al., 2009).
Target:CTP2-CP4 EPSPS

View other 257 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 241 328 + 88 bp
Amplicon: 88 bp 
1     gggatgacgt taattggctc tgagcttcgt cctcttaagg tcatgtcttc
51    tgtttccacg gcgtgcatgc ttcacggtgc aagcagcc

View other 233 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 22 bp 0 0 1 22 3.82e-10
primerB 100.0 19 bp 0 0 88 70 1.47e-08
probe 100.0 27 bp 0 0 65 39 8.22e-13

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.