Match details

Record ID: HW247719.1
Database: emblrelpat Go to original record
Description: JP 2013511293-A/3: Elite Event EE-GM3 and methods and kits for identifying such event in biological samples.
Taxon: synthetic construct
Original size: 1408 bp
Patents: JP2013511293 (lens, espacenet)

Method: QT-EVE-GM-001 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of soybean event FG72 (Savini et al., 2012)
Target:FG72

View other 41 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 191 260 + 70 bp

Amplicon: 70 bp
1     agatttgatc gggctgcagg aattaatgtg gttcatccgt ctttttgtta
51    atgcggtcat caatacgtgc  

View other 41 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 3.42e-09
primerB 100.0 22 bp 0 0 70 49 2.99e-10
probe 100.0 19 bp 0 0 25 43 1.15e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.