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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: A99126.1
Database: emblrelpat Go to original record
Description: Sequence 1 from Patent EP0899340.
Taxon: unidentified
Original size: 1117 bp
Patents: EP0899340 (lens, espacenet)
Method: QT-ELE-00-002 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of phosphinothricin N-acetyltransferase gene (Weighard et al., 2004).
Target:pat

View other 1579 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 371 483 + 113 bp
Amplicon: 113 bp 
1     ttgagggtgt tgtggctggt attgcttaca ccaaccaacg actccaactc
51    ccacaacacc gaccataacg aatggctggg ccctggaagg ctaggaacgc
101   ttacgattgg aca

View other 1 match with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 1.68e-09
primerB 100.0 21 bp 0 0 113 93 1.68e-09
probe 100.0 15 bp 0 0 89 75 3.87e-06

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.