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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: A95052.1
Database: emblrelpat Go to original record
Description: Sequence 9 from Patent WO9929880.
Taxon: unidentified
Original size: 4186 bp
Patents: WO9929880 (lens, espacenet)

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Method: QL-ELE-00-025 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative duplex PCR method for detection of pat gene; bar gene (partim pat) (Debode et al., 2016).
Target:pat

View other 1741 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 835 942 - 108 bp
Amplicon: 108 bp 
1     cgcggtttgt gatatcgtta accattacat tgagacgtct acagtgaact
51    ttaggacaga gccacaaaca ccacaagagt ggattgatga tctagagagg
101   ttgcaaga

View other 1557 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 22 bp 0 0 1 22 4.75e-10
primerB 100.0 25 bp 0 0 108 84 1.20e-11
probe 100.0 29 bp 0 0 53 81 8.17e-14

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.