Match details

Record ID: A31679.1
Database: emblrelpat Go to original record
Description: DNA sequence from patent DE4013099
Taxon: unidentified
Original size: 564 bp
Patents: DE4013099 (lens, espacenet)

View other 2 matches for this record


Method: QT-ELE-00-002 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of phosphinothricin N-acetyltransferase gene (Weighard et al., 2004).
Target:pat

View other 1579 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 197 264 + 68 bp

Amplicon: 68 bp
1     ttgagggtgt tgtggctggt attgcttacg ctgggccctg gaaggctagg
51    aacgcttacg attggaca 

View other 1562 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 9.83e-10
primerB 100.0 21 bp 0 0 68 48 9.83e-10
probe 100.0 22 bp 0 0 44 23 2.90e-10


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.