Match details

Record ID: HC043988.1
Database: emblrelpat Go to original record
Description: Sequence 1 from Patent EP2112224.
Taxon: synthetic construct
Original size: 291 bp
Patents: EP2112224 (lens, espacenet)

Method: QT-EVE-GM-005 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of soybean event GTS-40-3-2 (Mazzara et al., 2007).
Target:GTS 40-3-2

View other 18 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 76 159 - 84 bp

Amplicon: 84 bp
1     ttcattcaaa ataagatcat acatacaggt taaaataaac atagggaacc
51    caaatggaaa aggaaggtgg ctcctacaaa tgcc 

View other 17 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 31 bp 0 0 1 31 5.53e-15
primerB 100.0 21 bp 0 0 84 64 1.23e-09
probe 100.0 15 bp 0 0 63 49 1.86e-06


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.