Match details

Record ID: HB386790.1
Database: emblrelpat Go to original record
Description: Sequence 3 from Patent WO2009064652.
Taxon: synthetic construct
Original size: 6285 bp
Patents: WO2009064652 (lens, espacenet)

Method: QT-EVE-GM-010 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of soybean event MON87701 (Charels et al., 2011)
Target:MON87701

View other 11 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 5707 5795 + 89 bp

Amplicon: 89 bp
1     tggtgatatg aagatacatg cttagcatgc cccaggcacg cttagtgtgt
51    gtgtcaaaca ctgatagttt aaactgaagg cgggaaacg 

View other 11 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 28 bp 0 0 1 28 2.42e-13
primerB 100.0 22 bp 0 0 89 68 3.87e-10
probe 100.0 30 bp 0 0 64 35 2.03e-14


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.