Match details

Record ID: FW310535.1
Database: emblrelpat Go to original record
Description: PRODUCTION OF HIGH TRYPTOPHAN MAIZE BY CHLOROPLAST TARGETED EXPRESSION OF ANTHRANILATE SYNTHASE.
Taxon: synthetic construct
Original size: 13559 bp
Patents: JP2010500035 (lens, espacenet)

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Method: QL-ELE-00-019 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of CP4 epsps gene (Barbau-Piednoir et al., 2014).
Target:CP4-EPSPS

View other 281 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 11869 11976 + 108 bp

Amplicon: 108 bp
1     gcatgcttca cggtgcaagc agccggcccg caaccgcccg caaatcctct
51    ggcctttccg gaaccgtccg cattcccggc gacaagtcga tctcccaccg
101   gtccttca 

View other 165 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 18 bp 0 0 1 18 6.07e-08
primerB 100.0 19 bp 0 0 108 90 1.82e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.