Match details

Record ID: DJ056834.1
Database: emblrelpat Go to original record
Description: High Lysine Maize Compositions and Methods for Detection Thereof.
Taxon: synthetic construct
Original size: 1961 bp
Patents: JP2007513629 (lens, espacenet)

Method: QT-EVE-ZM-017 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of maize event LY038 (Charles Delobel et al., 2008).
Target:LYO38

View other 8 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1707 1817 + 111 bp

Amplicon: 111 bp
1     tgggttcagt ctgcgaatgt ttgggggtgg gggccaatcc ctgcgcagaa
51    tctcaggatc cgagcggagt ttatgggtcg acggtatcga taagcttgat
101   atcgaattcc t 

View other 8 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 1.65e-09
primerB 100.0 25 bp 0 0 111 87 1.24e-11
probe 100.0 24 bp 0 0 61 84 4.22e-11


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.