Match details

Record ID:	MP761926.1
Database:	emblrelpat	Go to original record
Description:	Sequence 89 from Patent WO2020169178.
Taxon:	synthetic construct
Original size:	1042 bp
Patents:	WO2020169178 (lens, espacenet)

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Method:	QT-EVE-BV-001	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Quantitative PCR method for detection of sugar beet event H7-1 (Mazzara et al., 2006).
Target:	H7-1

View other 21 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	242	349	+	108 bp

Amplicon:

108 bp

1     tgggatctgg gtggctctaa ctatttacat gagcctccgc gcgtttgctg
51    aaggcgggaa acgacaatct gatccccatc aagcttgagc tcaggattta
101   gcagcatt

View other 21 matches with an identical amplicon

Primers and probe alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	22 bp	0	0	1	22	4.75e-10
primerB	100.0	19 bp	0	0	108	90	1.82e-08
probe	100.0	20 bp	0	0	51	70	5.42e-09

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.