Match details

Record ID: LY410070.1
Database: emblrelpat Go to original record
Description: KR 1020160134709-A/10: TRANSGENIC MAIZE EVENT MON 87419 AND METHODS OF USE THEREOF.
Taxon: synthetic construct
Original size: 9259 bp
Patents: KR1020160134709 (lens, espacenet)

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Method: QT-EVE-ZM-029 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of maize event MON87419 (EURL GMFF, 2019).
Target:MON-87419-8

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1222 1318 + 97 bp

Amplicon: 97 bp
1     cggtcgctgc caggtattga tgtgcgccag tcagcatcat cacaccaaaa
51    gttaggcccg aatagtttga aattagaaag ctcgcaattg aggtctg 

View other 9 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 19 bp 0 0 1 19 1.62e-08
primerB 100.0 23 bp 0 0 97 75 1.25e-10
probe 100.0 26 bp 0 0 21 46 3.13e-12


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.