Match details

Record ID: LF938434.1
Database: emblrelpat Go to original record
Description: JP 2016187343-A/14: Herbicide tolerant soybean plants and methods for identifying same.
Taxon: synthetic construct
Original size: 810 bp
Patents: JP2016187343 (lens, espacenet)

Method: QT-EVE-GM-007 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of soybean event A5547-127 (Charles Delobel et al., 2009).
Target:A5547-127

View other 10 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 267 341 + 75 bp

Amplicon: 75 bp
1     gctatttggt ggcatttttc caaaaacccg caatgtcata ccgtcatcgt
51    tgtcagaagt aagttggccg cagtg 

View other 10 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 22 bp 0 0 1 22 3.23e-10
primerB 100.0 21 bp 0 0 75 55 1.09e-09
probe 100.0 26 bp 0 0 28 53 2.38e-12


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.