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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: JE980816.1
Database: emblrelpat Go to original record
Description: Sequence 2 from Patent EP2862934.
Taxon: synthetic construct
Original size: 9382 bp
Patents: EP2862934 (lens, espacenet)

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Method: QT-EVE-GH-001b Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of cotton event 3006-210-23 (Mazzara et al., 2006).
Target:DAS-21023-5

View other 12 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 508 597 + 90 bp
Amplicon: 90 bp 
1     aaatattaac aatgcattga gtatgatgtc cgggaaatct acatggatna
51    gcaatgagta tgatggtcaa tatggagaaa aagaaagagt

View other 6 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 28 bp 0 0 1 28 2.45e-13
primerB 100.0 26 bp 0 0 90 65 2.89e-12
probe 96.7 30 bp 1 0 60 31 2.52e-13

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.