Match details

Record ID: JD011421.1
Database: emblrelpat Go to original record
Description: Sequence 1 from Patent WO2014170387.
Taxon: synthetic construct
Original size: 415 bp
Patents: WO2014170387 (lens, espacenet)

Method: QT-EVE-BN-011 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of oilseed rape event MS11 (Jacchia et al., 2019).
Target:BCS-BN012-7

View other 13 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 152 275 - 124 bp

Amplicon: 124 bp
1     caagatggga attaacatct acaaattgcc ttttcttatc ggccatgtac
51    atcctaccat aattataatt ataattatat aatacttgaa accatggtgc
101   cccctgctgc tttacatgga tttc 

View other 6 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 28 bp 0 0 1 28 3.25e-13
primerB 100.0 23 bp 0 0 124 102 1.51e-10
probe 95.0 20 bp 1 0 40 59 2.91e-07


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.