Match details

Record ID:	JB987887.1
Database:	emblrelpat	Go to original record
Description:	Sequence 24 from Patent EP2602325.
Taxon:	synthetic construct
Original size:	5629 bp
Patents:	EP2602325 (lens, espacenet)

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Method:	QT-EVE-ZM-005	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Quantitative PCR method for detection of maize event MON87460 (Savini et al., 2011)
Target:	MON87460

View other 7 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	993	1074	+	82 bp

Amplicon:

82 bp

1     cacgttgaag gaaaatggat tggagggagt atgtagataa attttcaaag
51    cgttagacgg ctgtctttga ggaggatcgc ga

View other 7 matches with an identical amplicon

Primers and probe alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	22 bp	0	0	1	22	3.55e-10
primerB	100.0	20 bp	0	0	82	63	4.05e-09
probe	100.0	38 bp	0	0	24	61	8.41e-19

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.